Mixing plain agarose with 2,3-dibromopropanol under strongly alkaline conditions then desulfating the resulting crosslinked gel by alkaline hydrolysis, under reducing conditions, produces crosslinked polysaccharide chains with extremely low ionizable content. Crosslinked agarose beads offer the same selectivity, exclusion limits and fractionation ranges but they are chemically and physically more resistant as compared to plain agarose.
They also exhibit better flow characteristics and are resistant to organic solvents such as ethanol, dimethylformamide DMF ,tetrahydrofuran THF , acetone, chloroform, dichloromethane, dichloroethane, pyridine, triethyl phosphate and acetonitrile. Crosslinked agarose is resistant to biological degradation, is stable in aqueous solutions at pH 3 to14, and may be autoclaved repeatedly at o C and pH 7.
Image Source : Laurence Livermore. This assay is suitable for the simple and rapid estimation of protein concentration. This assay is based on a single Coomassie dye based reagent. The binding of protein to the dye results in a change of color from brown to blue. The change in color density is proportional to protein concentration. Inexpensive agarose for routine gels and standard analyses Good separation efficiency for nucleic acids of 1 to 20 kb length Easily melting, non-foaming agarose Low background fluorescence after ethidium bromide staining Very cost-effective.
Technical Information. Order form. Agarose Standard. Selected quantity: 0. Order No. German English French. You can search for and download your certificate of analysis for the selected product here. Please provide your batch number. The following analysis certificates have been found:. No certificate of analysis was found for the selected product. To make a request, please provide your e-mail address and the required language.
Your request has been sent. Order number:. Batch number:. E-mail address:. The agarose for IEF has no detectable electroendosmosis. Precast agarose gels have several advantages, in addition to speed and reproducibility. The locking tray means that the agarose gels do not move during loading and at the beginning of a run, which can waste sample, give uneven loads, and decrease band sharpness. The UV-transparent tray has fluorescent numbered wells that help keep track of loading patterns when there are many samples, as well as a fluorescent ruler down one side for measuring migration.
Well configurations suit needs from preparative to screening large numbers of samples up to four rows of wells. The uniformity makes precast agarose gels easy to load with a multichannel pipet. Precast agarose gels provide excellent separation and resolution of DNA, and are compatible with most submarine horizontal electrophoresis systems.
Agarose is a linear polysaccharide made of repeating units of agarobiose that is extracted from boiled red algae. It's neutral charge, low gelling temperature, and the formation of stable gels with large pore sizes makes agarose a good medium for electrophoresis and chromatography.
Agarose gels are easy and quick to set up, and can separate DNA fragments ranging from about 20 bp to many megabases. Agarose beads are used extensively for column chromatography, particularly in size exclusion and affinity chromatography. Certified Agarose Powders! Agarose Powders.
0コメント